|
KMID : 0364819870250040309
|
|
Korean Journal of Microbiology
1987 Volume.25 No. 4 p.309 ~ p.317
|
|
|
Induction of Bactericidal Substance from the Immunized Larval Haemolymph of L. illustris
|
|
|
|
|
Abstract
|
|
|
1
It was known that normal-haemolymph from the 3rd instar larvae of Lucilia illustris contain a lysozyme (or lysozyme-like substance) with bactericidal activity to gram positive bacteria, and the bactericidal activity of injured-haemolymph was increased significantly after injuring the body wall.
To elucidate the defence mechanism of insect against the nonpathogenic bacteria, the immunehaemolymph against Escherichia coil K-12 was prepared after immunization. The bactericidal activity between injured and immune-haemolymph was compared, and it was revealed that the immune-haemolymph showed higher titer of bactericidal activity to gram positive bacteria as well as to Escherichia coil.
The bactericidal substance from the immune-haemolymph was purified through a successive chromatographies on Sephacryl 5-300 and CM-Sepharose CL-6B, and it was characterized as a basic protein in nature with hat stable property at acidic conditions.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|